FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001321618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2854071
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1547175.420923305692115Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1136753.9829072227004865Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC616332.1594767614400623Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT535561.8764774947785112Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT432911.5168158045122213Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC334941.1735517441577312Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT325401.1401258062606012Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC261180.9151138846931278Clontech SMARTer II A Oligonucleotide (95% over 24bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN240760.8435669610181387No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA226340.7930426397941747Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA226130.7923068487083889Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC210570.7377882330187301Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG207530.7271367811102106Clontech Universal Primer Mix Long (96% over 25bp)
GCTTATACCTCTGCGTTGATACCACTGCTTATACCTCTGCGTTGATACCA180590.6327452961051073Clontech Universal Primer Mix Long (95% over 23bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC167640.5873715124816447Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC159670.5594464888925328Clontech Universal Primer Mix Long (96% over 25bp)
GCTTATACCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG151430.5305754481931249Clontech Universal Primer Mix Long (95% over 23bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA131950.4623220655687963Clontech Universal Primer Mix Long (96% over 25bp)
GGTATAAGCAGTGGTATCAACGCAGAGGTATAAGCAGTGGTATCAACGCA129870.45503423005244087Clontech Universal Primer Mix Long (95% over 22bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC123250.43183929201480975Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG118730.4160022648350374Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG114300.4004805766920304Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT111180.3895488234174973Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC111070.3891634090392285Clontech Universal Primer Mix Long (96% over 25bp)
GCTTATACCTCTGCGTTGATACCACTGCTTGTACCTCTGCGTTGATACCA107450.37647977222711Clontech Universal Primer Mix Long (95% over 24bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT105740.3704883305285678Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGG96160.33692224194843085Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGGTATAAGCAGTGGTATCAACGCAG83740.2934054548748087Clontech Universal Primer Mix Long (95% over 22bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG79860.27981083862314565Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG78070.2735390955585898Clontech Universal Primer Mix Long (96% over 25bp)
GCTTGTACCTCTGCGTTGATACCACTGCTTGTACCTCTGCGTTGATACCA73060.2559852225119838Clontech Universal Primer Mix Long (95% over 24bp)
GGTATAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG72700.2547238663649223Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTGTACCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG71860.2517807020217787Clontech Universal Primer Mix Long (95% over 23bp)
GGTATAAGCAGTGGTATCAACGCAGAGGTACAAGCAGTGGTATCAACGCA71700.25122009928975136Clontech Universal Primer Mix Long (95% over 22bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC64830.2271492194833275Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA62110.21761897303886274Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT60100.2105764012177693Clontech Universal Primer Mix Long (96% over 28bp)
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT59080.207002558801095Clontech Universal Primer Mix Long (96% over 27bp)
GCTTGTACCTCTGCGTTGATACCACTGCTTATACCTCTGCGTTGATACCA58640.2054609012880198Clontech Universal Primer Mix Long (95% over 23bp)
GAGTAAGCAGTGGTATCAACGCAGAGGTACAAGCAGTGGTATCAACGCAG58260.20412946979945487Clontech Universal Primer Mix Long (95% over 22bp)
GGTACAAGCAGTGGTATCAACGCAGAGGTACAAGCAGTGGTATCAACGCA56650.1984884048084298Clontech Universal Primer Mix Long (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTATACCTCTGCGTTGATACCACTG55550.19463426102574183Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT53090.18601499402082147Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT52990.1856646173133044Clontech Universal Primer Mix Long (96% over 25bp)
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA49060.1718948127078829Clontech Universal Primer Mix Long (96% over 25bp)
GGTACAAGCAGTGGTATCAACGCAGAGGTATAAGCAGTGGTATCAACGCA48710.1706684942315731Clontech Universal Primer Mix Long (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT46320.1622944909219147Clontech Universal Primer Mix Long (96% over 28bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA45130.15812500810246136Clontech Universal Primer Mix Long (96% over 25bp)
GCTTTCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT41660.14596693635161845Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA40740.14274347064246123Clontech Universal Primer Mix Long (96% over 25bp)
GAGGTATAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG38680.13552571046760925Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT38580.13517533376009216Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37150.1301649468425978No Hit
GCTTACTCTGCGTTGATACCACTGCTTGTACCTCTGCGTTGATACCACTG36700.12858825165877094Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG34440.12066973806888477Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGGTATAAGCAGTGGTATCAACGCAGAGGTATAAGCAGTGGTATCAACG33890.11874266617754078Clontech Universal Primer Mix Long (95% over 22bp)
GAGAAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33470.11727108400596901Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG30190.10577872799940856Clontech Universal Primer Mix Long (96% over 25bp)
GGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG29480.10329105337603725Clontech Universal Primer Mix Long (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGA29140.10209977257047914Clontech SMARTer II A Oligonucleotide (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)