FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001321626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2694830
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN229880.853040822612187No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT138300.5132049145957259Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT97940.36343665463127545Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC57300.2126293680862986Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT46610.1729608175654865Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT44020.16334982169561718Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCACTGCTTACTG41640.15451809576114264Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37490.1391182375140547No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC33000.12245670413347039Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT31680.11755843596813156Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA30840.11444135622655231Clontech Universal Primer Mix Long (96% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA30010.11136138457713474Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT29780.11050789845741661Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAA27090.10052582166593067No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)