FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001321643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3146574
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36718711.669422044420376Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2358647.495898713966365Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1428344.53934978169908Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1350044.290507707748173Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT1236283.9289716370884653Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC1005333.194998751022541Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT829502.6362005152270376Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC768692.442942705304245Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA719432.2863914848339815Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA643162.0440008720595797Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC547891.7412271251208458Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC516221.640577974647982Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG502291.596307603126448Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC420621.3367554680106044Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA345181.0970026447812764Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT331951.054956915044744Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC326011.036079240469158Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG313820.9973386928131994Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC290850.9243386616682144Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT288180.9158532422882791Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG265620.8441562156173668Clontech Universal Primer Mix Long (96% over 25bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG228890.7274260830986337Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTTCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT192640.6122214192324732Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG184290.5856846207970955Clontech Universal Primer Mix Long (96% over 25bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA178900.5685548790525823Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT171760.5458635328455647Clontech Universal Primer Mix Long (96% over 27bp)
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA167470.5322296567631971Clontech Universal Primer Mix Long (96% over 25bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA154300.4903746106082361Clontech Universal Primer Mix Long (96% over 25bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT148060.4705435181247922Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT145790.463329322622001Clontech Universal Primer Mix Long (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC145490.462375904714143Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT143210.45512992861442314Clontech Universal Primer Mix Long (96% over 28bp)
GAGAAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT116160.36916341392257107Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA115150.36595357363278286Clontech Universal Primer Mix Long (96% over 25bp)
GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT95480.3034411394742345Clontech Universal Primer Mix Long (96% over 28bp)
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG94110.29908719769501685Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGA88280.2805591096856454Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC83700.26600359629234843Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT83420.2651137395783478Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGGAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT79310.2520519142406948Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGAAAGCAGTGGTATCAACGCAGAGT77960.24776153365533432Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTTAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT69630.2212882964138139Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT62060.1972303845388667Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGC58800.18686990994014444Clontech Universal Primer Mix Long (96% over 28bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGT56410.17927434727421Clontech Universal Primer Mix Long (96% over 28bp)
GCTTACTCTGCGTTGATACCACTGCTTTCTCTGCGTTGATACCACTGCTT54600.1735220592301341Clontech Universal Primer Mix Long (96% over 25bp)
CAGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA54380.172822886097705Clontech Universal Primer Mix Long (100% over 25bp)
GTGTTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG53100.16875496969084472Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTAT52590.1671341592474863Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGT51520.16373363537612654Clontech SMART CDS Primer II A (95% over 24bp)
GTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT51020.16214460552969676Clontech Universal Primer Mix Long (96% over 28bp)
GCTTCCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT48040.15267398764497514Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAAGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT48020.15261042645111794Clontech SMART CDS Primer II A (100% over 26bp)
AGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG46780.14866963243197204Clontech Universal Primer Mix Long (96% over 28bp)
TATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC44370.1410105085721804Clontech Universal Primer Mix Long (96% over 28bp)
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC43480.13818203544553537Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATC42500.13506753694653295Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA40690.1293152489024571Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TTCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT39580.12578760264338293Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC37820.12019421758395005Clontech Universal Primer Mix Long (96% over 28bp)
CTGCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC36840.11707971908494763Clontech SMART CDS Primer II A (95% over 24bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG36580.11625342356480413Clontech SMART CDS Primer II A (100% over 26bp)
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG36540.11612630117708975Clontech Universal Primer Mix Long (96% over 25bp)
GCATTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG35900.1140923429736596Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCT34900.11091428328080001Clontech Universal Primer Mix Long (96% over 28bp)
CTGCTTACTCTGCGTTGATACCACTGCTTTCTCTGCGTTGATACCACTGC33010.10490775046129537Clontech Universal Primer Mix Long (96% over 25bp)
CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA32170.10223818031929331Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32130.10211105793157892Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGAAAGCAGTGGTAT31710.1007762728605779Clontech SMARTer II A Oligonucleotide (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)