FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001321663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2464700
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA312801.269119974033351Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT202740.8225747555483425Clontech Universal Primer Mix Long (96% over 26bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT170510.6918083336714408Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA169730.6886436483141964Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG153420.6224692660364345Clontech Universal Primer Mix Long (96% over 26bp)
GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA153320.62206353714448Clontech Universal Primer Mix Long (95% over 24bp)
GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT150310.6098510974966528Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT132780.5387268227370471Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA125070.5074451251673632Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG116230.4715786911185946Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT116010.4706860875562949Clontech Universal Primer Mix Long (96% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT111930.4541323487645555Clontech SMART CDS Primer II A (96% over 26bp)
GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA111340.4517385483020246Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA96230.39043291272771535Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG94020.3814663042155232Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT93000.37732786951758834Clontech Universal Primer Mix Long (96% over 26bp)
GCTTATACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA82700.33553779364628555Clontech Universal Primer Mix Long (96% over 26bp)
GCTTATACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC81380.33018217227248753Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC80680.3273420700288068Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA79890.32413681178236703Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC73860.2996713595975169Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA73700.2990221933703899Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC71970.2920030835395789Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA70170.2846999634843997Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA70150.28461881770600883Clontech Universal Primer Mix Long (100% over 24bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG69550.28218444435428247Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC66690.2705805980443868Clontech Universal Primer Mix Long (96% over 25bp)
GTATAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT66540.26997200470645516Clontech Universal Primer Mix Long (95% over 24bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC62620.2540674321418428Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA62570.25386456769586563Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT62020.25163305879011644Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT61750.25053759078183957Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA57860.23475473688481358Clontech Universal Primer Mix Long (96% over 26bp)
GCTTATACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA54950.22294802612894066Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA53410.21669980119284293Clontech Universal Primer Mix Long (96% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC52760.21406256339513938Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT50210.20371647665030226Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT48800.19799569927374527Clontech Universal Primer Mix Long (96% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGAT46550.18886679920477137Clontech Universal Primer Mix Long (96% over 26bp)
GCTTACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT45990.18659471740982675Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT45300.18379518805534142Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTATAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA44920.1822534182659147Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA43980.17843956668154337Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT43620.17697894267050757Clontech Universal Primer Mix Long (100% over 23bp)
GAGTATAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA42400.17202905018866393Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC40840.16569967947417535Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA40670.16500994035785288Clontech SMART CDS Primer II A (96% over 26bp)
GAGTAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA39870.1617641092222177Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39760.16131780744106788Clontech Universal Primer Mix Long (100% over 24bp)
GTATAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAAC39650.16087150565991803Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC39320.15953260031646854Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG39240.159208017202905Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGAT38810.15746338296750112Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA38280.1553130198401428Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC37970.15405526027508418Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA37630.15267578204243923Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG37330.15145859536657605Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATG37230.15105286647462166Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC37080.15044427313669007Clontech SMART CDS Primer II A (96% over 26bp)
GAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA36790.1492676593500223Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGG36680.14882135756887246Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC36680.14882135756887246Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTG35470.1439120379762243Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA35040.14216740374082038Clontech Universal Primer Mix Long (96% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG34720.1408690712865663Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34620.14046334239461192No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA34590.1403416237270256Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG34500.13997646772426664Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC34330.13928672860794417Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT33790.13709579259139043Clontech SMART CDS Primer II A (96% over 26bp)
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG33580.13624376191828622Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG32190.13060413032012008Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC31200.12658741428977158Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG30840.12512679027873577Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA30040.12188095914310058Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAAC29470.11956830445896051Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGG29180.11839169067229277Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTAC29160.1183105448939019Clontech SMART CDS Primer II A (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGT29150.11826997200470644Clontech Universal Primer Mix Long (96% over 26bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT29130.11818882622631557Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC28850.11705278532884328Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAATACATGGGAAGCAGTGGTA28770.11672820221527976Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCTTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGC28690.11640361910171623Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG28660.1162819004341299Clontech Universal Primer Mix Long (96% over 26bp)
GCTTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC28450.11542986976102569Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC28240.11457783908792145Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTG28070.11388809997159897Clontech Universal Primer Mix Long (96% over 26bp)
GCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGT28040.11376638130401266Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG27760.11263034040654035Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC27650.11218403862539052Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA27440.11133200795228627Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTCC26700.10832961415182375Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGG26640.10808617681665111Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC26510.10755872925711041Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC26200.10630096969205177Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC26020.10557065768653384Clontech Universal Primer Mix Long (96% over 26bp)
GTACAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAAC25690.10423175234308435Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTATAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCA25310.10268998255365766Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA25220.10232482655089868Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph