FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001321685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2476217
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83900.3388232937581803No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67390.27214900794235725No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36980.14934070802356983Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32870.13274280888952786No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG29670.1198198703910037No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT26600.10742192626898209No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)