FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001321686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2476217
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN216520.874398326156391No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84440.34100403962980624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62870.25389535731319185No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33140.13383318182534082Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31140.12575634526376325No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG26090.10536233294577979No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)