FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001321695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2561780
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT74030.2889787569580526Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54610.2131720912802817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40230.1570392461491619No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT38450.15009095238467002Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT31920.124600863462124Clontech Universal Primer Mix Long (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT25740.10047701207753984No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)