FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001321724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2292728
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN192940.8415302643837386No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98740.4306660013747815No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT79740.34779529015216804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78480.34229965351319475No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA72300.3153448642839447No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA66370.289480479149729No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA64090.2795359938030154No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA60630.26444480112773955No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG60280.2629182353946914No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC56550.24664940629677834No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG54600.23814425435551012No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA52050.22702213258615936No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT49170.21446067741136324No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC47320.20639168710810876No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG47250.20608637396149912No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT45980.20054712115872447No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTAT45630.1990205554256763No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC45370.19788653516684054No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC41770.18218471619834536No Hit
GTGTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTT39000.17010303882536437No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT38770.16909986705793273No Hit
GTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGAT38610.16840200843711073No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38260.1668754427040626Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37830.16499994766060344No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG36470.15906814938361635No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT36350.15854475541799987No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA35550.15505546231388984No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC35160.15335443192563616No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG34340.1497779064939234No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA34330.14973429033012203No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC33340.14541629011378585No Hit
TGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTG32640.14236315864768956No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT32510.1417961485182717No Hit
GTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAAC32430.14144721920786069No Hit
ATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA32120.14009511813001804No Hit
GTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAG31900.13913556252638778No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC31180.13599519873268875No Hit
CAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCT30470.13289845110279108No Hit
TTCCTTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACT29340.12796982459323566No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG28950.126268794204982No Hit
GGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTT28460.12413160217871462No Hit
CATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGT28280.12334651123028985No Hit
GTACATGGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATAT28230.12312843041128298No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28100.1225614202818651Clontech Universal Primer Mix Long (96% over 25bp)
CCACTATGCTTAGCCCTAAACCTCAACAGTTAAACCAACAAAACTGCTCG28030.12225610713525548No Hit
GTACATGGGGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCC27650.1205986929108032No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA27510.11998806661758395No Hit
ACTTATACTAACATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTGG27410.1195519049795702No Hit
TAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACAC26710.1164987735134739No Hit
ACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATAAGGG26440.11532113709083677No Hit
ACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTG26370.11501582394422714No Hit
TACTTATACTAACATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTG26130.11396903601299413No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA25910.11300948040936386No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT25840.11270416726275424No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT25610.1117009954953226No Hit
CTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAAC25450.11100313687450059No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTA25190.10986911661566483No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA25050.10925849032244557No Hit
CTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC24900.10860424786542494No Hit
GGGTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAG24820.10825531855501394No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA24620.10738299527898643No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT24620.10738299527898643No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG24480.10677236898576717No Hit
TTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTC24410.10646705583915754No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG24010.10472240928710254No Hit
ATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCA23750.10358838902826677No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA23410.10210543945902No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG23140.10092780303638287No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT22940.10005547976035536Clontech Universal Primer Mix Long (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)