FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001321727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2463859
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82610.3352870436173499No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66960.27176879845802865No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG46920.1904329752636007No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT41030.1665273865103482No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG40880.16591858543853363No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG40320.16364572810375919No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT40200.16315868724630753No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA37040.15033327800008037No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA35550.14428585402005553No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34680.14075480780353095No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA33200.1347479705616271No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA32630.1324345264887317No Hit
GCTCTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTT31880.1293905211296588No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT31390.12740177096173116No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG31390.12740177096173116No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC29960.12159786741043217No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC29540.11989322440935134No Hit
GTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGAT28750.11668687209779455No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC28480.11559103016852831No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT28060.11388638716744749No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA27470.11149176961831014No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC27350.11100472876085848No Hit
TCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTA27300.11080179507025362No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA27200.1103959276890439No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA27150.11019299399843904No Hit
TTACTATTCTGCCTAGCAAACTCAAACTACGAACGCACTCACAGTCGCAT26410.1071895753774871No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT26370.1070272284250032No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA26090.10589079975761599No Hit
GCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAAT25090.1018321259455188No Hit
GATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTAC24960.10130449834994616No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)