FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001321737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1228942
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57020.46397633086020335No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49100.3995306531960011Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49080.39936791158573803No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25590.20822789033168365No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT23830.19390662862852762No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20570.1673797461556363Clontech Universal Primer Mix Long (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT20410.16607781327353122RNA PCR Primer, Index 26 (100% over 22bp)
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT19470.1584289575911638No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA17700.14402632508287616No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16740.1362147277902456Clontech Universal Primer Mix Long (96% over 25bp)
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA16540.13458731168761423No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT15200.12368362379998406Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA14850.12083564562037916No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC14800.12042879159472131Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC14620.11896411710235308Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT13730.11172211544564349RNA PCR Primer, Index 26 (100% over 24bp)
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT12950.10537519264538116No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG12950.10537519264538116No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA12860.10464285539919704No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT12810.1042360013735392No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC12660.10301543929656568Clontech Universal Primer Mix Long (96% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)