FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001321755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2464833
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106020.4301305605694179No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88070.3573061542100418No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT87970.35690044721082526Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG53110.21547098728392552No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT47400.19230511762865882Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45490.18455611394362215No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT42290.1715734899686916No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT38420.15587262909900995Clontech Universal Primer Mix Long (96% over 25bp)
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG35920.14572995411859546No Hit
GCTCTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTT34450.13976606123011173No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA32200.1306376537477387No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA30800.12495775575870657No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT29580.1200081303682643Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG28090.11396309607993725No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA27760.11262426298252255No Hit
GATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTAC27760.11262426298252255No Hit
GAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCG27300.11075801078612628No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC27270.1106362986863613No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT27210.11039287448683137No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC27170.11023059168714473Clontech Universal Primer Mix Long (96% over 25bp)
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA26400.10710664779317706No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA26210.10633580449466556No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC26150.10609238029513561Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGT25630.1039827038992094No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG25560.1036987089997578No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA24780.10053419440586846No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)