FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001321800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421894
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN124740.8772805849099863No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104130.7323330712415974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75430.5304896145563593No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43120.30325748614172365No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG28430.19994458096032475No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA18230.12820927579693No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG17500.12307527846660861No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT17370.1221610049694281No Hit
GCTCTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTT16770.11794128113628724No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG16190.11386221476425105No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG15310.10767328647564446No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA15190.10682934170901628No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14500.10197665930090427Clontech SMART CDS Primer II A (100% over 21bp)
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA14430.10148435818703785No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA14380.10113271453427611No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)