FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001321823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4515748
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT89890.19905893774409023Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72600.16077070731139115No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54450.12057803048354336No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG45530.10082493531525674No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)