FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001321833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245751
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72590.5827007162747612No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57610.4624519667252926No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT51510.4134855199795144No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG31960.25655207180247097TruSeq Adapter, Index 10 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30650.2460363266816563No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT30240.24274513927743185TruSeq Adapter, Index 10 (95% over 21bp)
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT30230.24266486641391416No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG27940.22428238066836792No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA27830.22339937916967356No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA27720.2225163776709792No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT23220.18639358908802803No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT21790.17491456960500132No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTAT21080.1692151962952468No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA20940.16809137620599943No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA20180.16199063857865656No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT19960.16022463558126784RNA PCR Primer, Index 10 (95% over 23bp)
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC18820.15107352914025357No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC16980.1363033222530024No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT16720.134216227801543No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG16020.12859712735530615No Hit
ATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA15990.12835630876475315No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT15770.1265903057673644No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA15320.12297802690906931No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA15290.1227372083185163No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG14900.11960656664132721No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG14090.113104464696396No Hit
CATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGT13630.1094119129745832No Hit
GTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAAC13380.10740509138664146No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG13340.1070839999325708No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC13270.1065220898879471No Hit
GGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTT13110.1052377240716644No Hit
GTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAG12940.10387308539186403No Hit
GATAGAAACCGACCTGGATCACTCCGGTCTGAACTCAGATCACGTAGGAC12930.10379281252834635No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC12720.10210708239447529No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTT12520.1005016251241219No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)