FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001321839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3278339
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTC144430.4405584657352397No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG103980.31717281220764537No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98120.2992979066533388No Hit
GTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAACGTCTGC91410.27883022469610375No Hit
GAGTACATGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT87630.26729999551602196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82410.2513772980768615No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT75950.23167219741460537No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT65580.2000403252988785Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT60940.18588681646406915No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA57380.17502765882356888No Hit
ACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATC55450.16914053122633138No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT54660.1667307743341979No Hit
TATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAACGTCTGCC53450.1630398808664998No Hit
GGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAACGTCTG52300.1595320069095966No Hit
ACATGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGA43900.13390927539830383No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT39570.1207013673692684No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT38990.11893217876491724No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAG38740.11816959746993828No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT38570.1176510421893526No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAG36710.11197743735470919No Hit
ATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGAC36300.11072680403094372No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC35430.10807302112441697No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC35330.10776798860642539No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT34560.10541923821789023Clontech Universal Primer Mix Long (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)