FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001321847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3914364
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT166310.4248710646225032No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC128360.32792044889029226No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT119310.304800473333599No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC104030.26576475769754676No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103840.2652793659455278No Hit
TTATTATTGTCAGCAGTACGATAGGTGGCCGCTCACCTTCGGCGCAGGGA100860.25766637951912497No Hit
GTCTCCAGGCACCCTGTCTGTGTCTCCAGGGGGCAGCGCCACCCTCTCTT99850.25508613915312933No Hit
CTCCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGA98530.2517139438233133No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG97070.24798409141306224No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG91510.2337799959329281No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC90970.23240046147982152No Hit
GTACGATAGGTGGCCGCTCACCTTCGGCGCAGGGACCAAGGTGCAGATCA90940.2323238206768711No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG90700.23171069425326823No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTACAGTCTGACGATT87040.22236051629332376No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG83690.21380229329719977No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGA79340.20268937686939692No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76560.19558732912932983No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC73770.18845973445494593No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA72330.18478097591332843No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT71520.18271167423366863No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC68890.17599283050835332No Hit
CCTATCGTACTGCTGACAATAATAAACTGCAAAATCGTCAGACTGTAGGC64320.1643178815255812No Hit
CTCTTGCAGGGCCAGTCAGACTCTCAGAGGTAACTTAGCCTGGTACCAAC64190.16398577137946294No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA63590.16245295532045564No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA61510.1571391929825637No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT59290.15146777356423674No Hit
GTCTGTGTCTCCAGGGGGCAGCGCCACCCTCTCTTGCAGGGCCAGTCAGA57800.14766128035103532No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC57740.1475079987451346No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG57670.14732917020491706No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC56390.1440591626123682No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC54720.13979282458146455No Hit
GCCTACAGTCTGACGATTTTGCAGTTTATTATTGTCAGCAGTACGATAGG54060.13810672691655657No Hit
CTCCAGGCACCCTGTCTGTGTCTCCAGGGGGCAGCGCCACCCTCTCTTGC53320.13621625377711424No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT53170.13583304976236243No Hit
AACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCAT52960.13529656414170987No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA52690.1346067969151566No Hit
GCCCTGGAGGATGCACCAAAGATGAGGAGCCTGGGAGCCTGGCCAGGTTT51970.13276741764434782No Hit
CAATAATAAACTGCAAAATCGTCAGACTGTAGGCTGCTGATGGTGAGAGT50510.1290375652340968No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA50470.12893537749682962No Hit
TCTTTGGTGCATCCTCCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGC49970.12765803078099022No Hit
CCTCTGGAGAAACAGTGTTGACGCAGTCTCCAGGCACCCTGTCTGTGTCT49250.12581865151018148No Hit
GAGTTCACTCTCACCATCAGCAGCCTACAGTCTGACGATTTTGCAGTTTA47920.12242090924604865No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTGCACCTTGGTCC47010.12009613822322093No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG46980.12001949742027058No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG45810.11703050610520635No Hit
GTGTTGACGCAGTCTCCAGGCACCCTGTCTGTGTCTCCAGGGGGCAGCGC45690.11672394289340492No Hit
GTGCAGCCACAGTTCGTTTGATCTGCACCTTGGTCCCTGCGCCGAAGGTG45450.11611081646980198No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA45000.11496120442554654No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG44380.11337729449790568No Hit
GTTTATTATTGTCAGCAGTACGATAGGTGGCCGCTCACCTTCGGCGCAGG44230.11299409048315383No Hit
ATAATAAACTGCAAAATCGTCAGACTGTAGGCTGCTGATGGTGAGAGTGA44100.11266198033703559No Hit
ATAGGTGGCCGCTCACCTTCGGCGCAGGGACCAAGGTGCAGATCAAACGA43600.11138463362119619No Hit
ATTGTCAGCAGTACGATAGGTGGCCGCTCACCTTCGGCGCAGGGACCAAG43210.11038830318284146No Hit
GATTCACTTTATTTATTCATTCTCCTCCAACATTAGCATAATTAAAGCCA41340.10561102646560207No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA41180.10520227551653347No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG40770.10415485120954515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40330.10303078609960646No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA40300.10295414529665611No Hit
GTACATGGGGGAGGAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAG40270.10287750449370575No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC39320.10045054573361088No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA39240.10024617025907658No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)