FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001321848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3914364
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN328150.8383226496054021No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT160190.4092363408206288No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC117220.2994611640613903No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT106420.2718704749992591No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC104310.2664800718584168No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97310.24859721783666516No Hit
TTATTATTGTCAGCAGTACGATAGGTGGCCGCTCACCTTCGGCGCAGGGA93130.2379185992922477No Hit
CTCCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGA92690.23679453418230906No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG91190.23296249403479083No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGA89960.22982022111382588No Hit
GTACGATAGGTGGCCGCTCACCTTCGGCGCAGGGACCAAGGTGCAGATCA88040.22491520972500256No Hit
GTCTCCAGGCACCCTGTCTGTGTCTCCAGGGGGCAGCGCCACCCTCTCTT85290.21788980278788583No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG84350.21548839096210776No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTACAGTCTGACGATT78540.20064562212405387No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA72060.18409120868677517No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC71840.18352917613180583No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG71680.18312042518273722No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG71470.18258393956208469No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT70950.18125549897761167No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC69890.17854752394003215No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65320.16687257495726No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC64540.1648799140805505No Hit
CCTATCGTACTGCTGACAATAATAAACTGCAAAATCGTCAGACTGTAGGC62020.15844208663271991No Hit
CTCTTGCAGGGCCAGTCAGACTCTCAGAGGTAACTTAGCCTGGTACCAAC58910.15049699006019882No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA57990.1481466721030543No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC57920.14796784356283676No Hit
GCCCTGGAGGATGCACCAAAGATGAGGAGCCTGGGAGCCTGGCCAGGTTT57520.14694596619016523No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT56980.1455664317370587No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT56340.14393142794078426No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG54430.1390519634862777No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA52050.13297179311888213No Hit
GCCTACAGTCTGACGATTTTGCAGTTTATTATTGTCAGCAGTACGATAGG51770.13225647895801207No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA51000.13028936501561938No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA50270.12842443881049387No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC50010.1277602185182574No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC50010.1277602185182574No Hit
CAATAATAAACTGCAAAATCGTCAGACTGTAGGCTGCTGATGGTGAGAGT48870.12484786800614353No Hit
GTCTGTGTCTCCAGGGGGCAGCGCCACCCTCTCTTGCAGGGCCAGTCAGA47990.12259973778626616No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC47150.12045379530365596No Hit
CTCCAGGCACCCTGTCTGTGTCTCCAGGGGGCAGCGCCACCCTCTCTTGC47080.12027496676343845No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG46690.11927863632508372No Hit
AACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCAT45620.1165451143531874No Hit
TCTTTGGTGCATCCTCCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGC44430.1135050291694896No Hit
GAGTTCACTCTCACCATCAGCAGCCTACAGTCTGACGATTTTGCAGTTTA43760.1117933845702648No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG43530.11120580508097867No Hit
GTGCAGCCACAGTTCGTTTGATCTGCACCTTGGTCCCTGCGCCGAAGGTG43480.11107807040939473No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTGCACCTTGGTCC43220.11041385011715825No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG42880.10954525435038745No Hit
ATAATAAACTGCAAAATCGTCAGACTGTAGGCTGCTGATGGTGAGAGTGA42120.10760368734231156No Hit
CCTCTGGAGAAACAGTGTTGACGCAGTCTCCAGGCACCCTGTCTGTGTCT42060.10745040573641082No Hit
GTTTATTATTGTCAGCAGTACGATAGGTGGCCGCTCACCTTCGGCGCAGG42060.10745040573641082No Hit
ATTGTCAGCAGTACGATAGGTGGCCGCTCACCTTCGGCGCAGGGACCAAG41320.1055599325969685No Hit
TCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT40590.10369500639184295No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG40350.10308187996824006No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA39670.10134468843469846No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA39310.10042499879929409No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)