FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001321855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542358
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT77691.4324486778105974No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT46210.8520202523056726RNA PCR Primer, Index 46 (95% over 21bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30270.5581184383746529Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG28500.5254831679444205RNA PCR Primer, Index 46 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT28310.521979946824791RNA PCR Primer, Index 46 (95% over 23bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17520.3230338632416227Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15670.28892355233996736No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13640.25149440037761034No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12870.23729713584016462Clontech Universal Primer Mix Long (96% over 25bp)
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT11660.21498714870989274RNA PCR Primer, Index 46 (96% over 26bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC9560.17626733633504071Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT9260.17073593456720468Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC9180.16926089409578174Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC8850.16317635215116214Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG8540.15746057032439828TruSeq Adapter, Index 4 (95% over 23bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA6920.12759100077808386Clontech Universal Primer Mix Long (96% over 25bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT6760.12464091983523798Clontech Universal Primer Mix Long (96% over 25bp)
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA6610.12187521895131999RNA PCR Primer, Index 46 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6430.11855637789061836No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC6310.11634381718348397Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG6280.11579067700670037Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA5830.10749357435494637Clontech Universal Primer Mix Long (96% over 25bp)
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC5540.10214655264603822No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)