FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001321868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213953
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21741.0161110150360126No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19990.9343173500722122No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8210.3837291367730296No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5440.254261449944614No Hit
GCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTG5190.24257664066407109No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT3890.1818156324052479No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3560.16639168415493122No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG3520.16452211467004435No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3410.15938079858660548No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3310.1547068748743883Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG3100.14489163507873226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3080.1439568503362888No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2750.12853290208597215No Hit
GTGTTAAGAAGAGGAAATGAACCTCTGACTGTAAAGTTTTAAGTTTTATG2660.12432637074497671No Hit
GTATCAACGCAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGG2590.12105462414642469No Hit
GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG2500.11684809280542922No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC2490.1163807004342075No Hit
ACACAAAGCACCCAACTTACACTTAGGAGATTTCAACTTAACTTGACCGC2470.11544591569176409No Hit
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTAAGTGTAAGTTGGGTGC2410.11264156146443378No Hit
GGTATCAACGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTC2400.11217416909321207No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT2400.11217416909321207No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC2380.11123938435076863No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT2380.11123938435076863No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT2350.10983720723710347No Hit
AACTTACACTTAGGAGATTTCAACTTAACTTGACCGCTCTGACCAAAAAA2310.1079676377522166No Hit
GTATCAACGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTCA2250.10516328352488631No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT2230.10422849878244286No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)