FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001321871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32099
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT677621.109691890713105No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT332310.35234742515343TruSeq Adapter, Index 11 (95% over 21bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG329310.258886569675067TruSeq Adapter, Index 11 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT20806.479952646499891RNA PCR Primer, Index 11 (95% over 23bp)
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA13064.068662575158104RNA PCR Primer, Index 11 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT8522.6542882955855323TruSeq Adapter, Index 11 (96% over 26bp)
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG5681.7695255303903548TruSeq Adapter, Index 11 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC4151.2928751674506995No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT3381.0529923050562324No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGC2490.7757251004704196RNA PCR Primer, Index 17 (95% over 24bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGTACTA2200.6853796068413346No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC1210.37695878376273406No Hit
ATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTT1130.3520358889685037TruSeq Adapter, Index 11 (96% over 27bp)
TCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAA990.3084208230786006TruSeq Adapter, Index 11 (96% over 28bp)
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACCGTACTA960.2990747375307642No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATATCGTATGCCGT950.29595937568148545No Hit
CTTTACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC800.24922894794230352No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGT550.17134490171033365No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGTACT540.16822953986105485No Hit
TCTCCGAGCCCACGAGACCGTACTAGATATCGTATGCCGTCTTCTGCTTG520.16199881616249726Illumina PCR Primer Index 9 (96% over 26bp)
CTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC440.13707592136826693No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.13084519766970934No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.12149911212187296No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATATCGTATGCCGTCTT370.11526838842331538No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGT340.10592230287547898No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)