FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001321873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136913
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT66384.848334343707317No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT30432.222579302184599TruSeq Adapter, Index 11 (95% over 21bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG21271.553541299949603TruSeq Adapter, Index 11 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT18471.349031866952006RNA PCR Primer, Index 11 (95% over 23bp)
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA9210.6726899563956673RNA PCR Primer, Index 11 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT7050.5149255366546639TruSeq Adapter, Index 11 (96% over 26bp)
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG4780.3491268177601835TruSeq Adapter, Index 11 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC3440.2512544462541906No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT3230.23591623877937085No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGC2160.15776441974100341RNA PCR Primer, Index 17 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC1870.13658308560910945No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)