FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001321884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76207
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15532.0378705368273256No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11501.509047725274581No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7290.9566050362827561No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6150.8070124791685803No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2550.3346149303869723No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.3162439146010209No Hit
GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2320.3044339758814807No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2250.29524846798850496No Hit
GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA2160.2834385292689648No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1910.25063314393690866No Hit
GAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1760.230949912737675No Hit
GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA1740.22832548191111052No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1680.22045218943141706No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1660.2178277586048526Clontech SMART CDS Primer II A (100% over 21bp)
GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1620.2125788969517236No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1520.19945674281890116No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1510.1981445274056189No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC1450.19027123492592543No Hit
GTACATGGGATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACC1360.17846129620638526No Hit
GTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA1360.17846129620638526No Hit
TATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1330.1745246499665385No Hit
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1320.17321243455325627No Hit
GGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA1250.16402692666028057No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC1210.15877806500715158No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC1170.1535292033540226No Hit
GTACATGGGAGCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTCG1110.14565591087432914No Hit
ACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1070.14040704922120015No Hit
ATATAATACTGGAACTACAGTTAAAAACAAACAAACCAATCTTTGAGGAT1070.14040704922120015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1050.13778261839463565No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC1040.13647040298135343No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.13515818756807116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC1020.13384597215478894No Hit
GTACATGGGGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTT980.12859711050165995No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC930.12203603343524873No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT910.11941160260868423No Hit
TATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA890.11678717178211975No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.11416274095555526No Hit
GGATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAACCAC860.11285052554227303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC840.11022609471570853No Hit
GTACATGGGAAGGCCTGAGAGTTTTTGCAGAAATGGGGCAGAGGGACACC830.1089138793024263No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG830.1089138793024263No Hit
GTACATGGGATAGAAAGTTGGTACAGATGATCTGCACTGTCAAGTAAACT780.10235280223601508No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)