FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001321887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42321
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT825019.493868292337137No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT38159.014437277001962RNA PCR Primer, Index 17 (95% over 21bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG24985.902507029607051RNA PCR Primer, Index 17 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT22955.42283972495924RNA PCR Primer, Index 17 (95% over 23bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT9162.164410103731008RNA PCR Primer, Index 17 (96% over 26bp)
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA8662.0462654474138136RNA PCR Primer, Index 17 (96% over 28bp)
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG6271.4815339902176226TruSeq Adapter, Index 12 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC4831.1412773800241016No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT3990.9427943574112143No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGC2790.6592471822499468TruSeq Adapter, Index 25 (95% over 24bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAGGCAGA2600.6143522128494128No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA1820.430046548994589TruSeq Adapter, Index 25 (96% over 27bp)
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC1710.4040547246048061No Hit
ATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTT1070.2528295645187968TruSeq Adapter, Index 12 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATATCGTATGCCGT1010.23865220576073345No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACAGGCAGA910.2150232744972945No Hit
CTTTACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC850.2008459157392311No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAGGCAG690.1630396257177288No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAACTCGTATGCCGT690.1630396257177288No Hit
CTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC680.1606767325913849No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.12523333569622647No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAGCTCGTATGCCGT480.11341887006450699No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)