FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001321888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42321
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTGGTC8391.9824673330025282No Hit
CATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTGGTCGCCGT4731.1176484487606626Illumina RNA PCR Primer (96% over 25bp)
ATACACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTGGGC1280.3024503201720186No Hit
CATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTGGTCGCCGG1030.24337799201342125Illumina RNA PCR Primer (95% over 24bp)
TATACACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTGGT730.17249119822310435No Hit
CATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTGGGCGCCGT690.1630396257177288Illumina Single End PCR Primer 1 (95% over 23bp)
ATACACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGGGGTC560.13232201507525815No Hit
CACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTGGTCGCC540.12759622882257035Illumina RNA PCR Primer (95% over 23bp)
CATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTGGTCGCCGC460.10869308381181919Illumina RNA PCR Primer (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)