FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001321889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36713
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT779121.22136572876093No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT34589.419006891291913RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT20115.4776237300138915RNA PCR Primer, Index 17 (95% over 23bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG19205.229755127611472RNA PCR Primer, Index 17 (96% over 28bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT7522.0483207583144933RNA PCR Primer, Index 17 (96% over 26bp)
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA6431.7514232015907172RNA PCR Primer, Index 17 (96% over 28bp)
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG5841.5907171846484898TruSeq Adapter, Index 12 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC4321.1766949037125813No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT3160.8607305314193882No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGC2480.675510037316482TruSeq Adapter, Index 25 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC2130.5801759594693977No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAGGCAGA1870.5093563587829925No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA1690.4603274044616348TruSeq Adapter, Index 25 (96% over 27bp)
ATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTT830.22607795603737096TruSeq Adapter, Index 12 (96% over 26bp)
CTTTACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC750.20428730967232317No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATATCGTATGCCGT720.19611581728543023No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAACTCGTATGCCGT630.17160134012475145No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACAGGCAGA620.1688775093291205No Hit
CTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC620.1688775093291205No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAGGCAG530.1443630321684417No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAC500.13619153978154877TruSeq Adapter, Index 25 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.1225723858033939No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)