FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001321890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36713
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC7492.0401492659276004No Hit
CATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCGCCGT4001.0895323182523902Illumina RNA PCR Primer (96% over 25bp)
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGGC1670.4548797428703729No Hit
CATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCGCCGG1050.28600223354125237Illumina RNA PCR Primer (95% over 24bp)
CATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGGCGCCGT960.26148775638057364No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGGGGTC930.2533162639936807No Hit
TATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGT560.15253452455533464No Hit
CATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGGCGCCGG500.13619153978154877No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.11712472421213194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.10078173943834608No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)