FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001321891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215508
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT73233.3980177069992763No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT33561.5572507749132283RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT20100.9326799933181135RNA PCR Primer, Index 17 (95% over 23bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG19570.9080869387679343RNA PCR Primer, Index 17 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8810.40880152940958103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7500.3480149228798931No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT7390.342910704010988RNA PCR Primer, Index 17 (96% over 26bp)
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA6850.3178536295636357RNA PCR Primer, Index 17 (96% over 28bp)
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG5130.23804220724984687TruSeq Adapter, Index 12 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5080.23572210776398092No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC3730.17307942164560017No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3010.13966998904913044No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT2850.13224567069435939No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)