FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001321894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382820
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC9490.24789718405516956No Hit
CATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTCGCCGT5300.13844626717517372Illumina Single End PCR Primer 1 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5190.1355728540828588No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4390.11467530432056841No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)