FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001321907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46784
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT818617.497435020519838No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT40398.63329343365253RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT23785.082934336525308RNA PCR Primer, Index 26 (100% over 24bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG21924.685362517099864RNA PCR Primer, Index 26 (100% over 29bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT8881.8980848153214775RNA PCR Primer, Index 26 (100% over 27bp)
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA7631.6308994528043776RNA PCR Primer, Index 26 (100% over 29bp)
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG6571.404326265389877RNA PCR Primer, Index 26 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC4310.9212551299589602RNA PCR Primer, Index 26 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT3580.765218878248974RNA PCR Primer, Index 26 (100% over 21bp)
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC2630.5621580027359782RNA PCR Primer, Index 26 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC2030.4339090287277702No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCCTGAG1830.3911593707250342No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.2009233926128591No Hit
ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTT930.1987859097127223RNA PCR Primer, Index 26 (96% over 30bp)
CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC880.1880984952120383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.1859610123119015No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACTCCTGAG790.1688611491108071No Hit
CTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC590.12611149110807113RNA PCR Primer, Index 26 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGT580.12397400820793433No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCCTGA580.12397400820793433No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)