FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001321923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149212
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT101056.7722435192879935No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT50393.3770742299546956RNA PCR Primer, Index 27 (95% over 23bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG32962.208937618958261RNA PCR Primer, Index 27 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT28881.9355011661260488RNA PCR Primer, Index 27 (96% over 25bp)
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA13870.9295499021526419RNA PCR Primer, Index 27 (96% over 30bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT10490.7030265662279174RNA PCR Primer, Index 27 (96% over 28bp)
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG7230.4845454789159049RNA PCR Primer, Index 27 (100% over 24bp)
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC5810.3893788703321449No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT4820.32303031927726994RNA PCR Primer, Index 27 (95% over 22bp)
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGC3620.24260783315014878TruSeq Adapter, Index 27 (96% over 25bp)
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC2760.1849717180923786No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGGACTCC2340.15682384794788623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2000.13403747687853523No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1940.1300163525721792No Hit
GGTATAGACAATGTACACGAGTTGTTTAGTGTATTTATGTGTCTGCTTTA1520.10186848242768679No Hit
TTATTAGAGTCTGTGATGGCAGGGCCTCCTAGGGAAAGCAGCAGAAGGAA1510.10119829504329411No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)