FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001321927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3299164
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT97140.2944382273812396No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT49950.1514019915348252RNA PCR Primer, Index 27 (95% over 23bp)
GTACATGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGAC44930.13618601560880272No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG40720.1234252071130747RNA PCR Primer, Index 27 (96% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40670.1232736535679948No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35420.10736053133460477No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)