FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001321929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116289
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT109069.378359088133873No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT43833.769058122436344RNA PCR Primer, Index 27 (95% over 23bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG27312.3484594415636906RNA PCR Primer, Index 27 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT26092.2435484009665574RNA PCR Primer, Index 27 (96% over 25bp)
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA12091.0396512137863425RNA PCR Primer, Index 27 (96% over 30bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT9600.8255294997807188RNA PCR Primer, Index 27 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9600.8255294997807188No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9230.7937122169766702No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG5730.49273792018161655RNA PCR Primer, Index 27 (100% over 24bp)
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC5360.460920637377568No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4340.3732081280258666No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT3850.33107172647455907RNA PCR Primer, Index 27 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC3480.2992544436705105No Hit
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGC2830.24335921712285769TruSeq Adapter, Index 27 (96% over 25bp)
CACATATATCATGCAAGGTACAAATTTAAAAACATGTTACCACTAGATAC2270.19520332963564913No Hit
GTCTTTAATCGTGGCAGGGCCTCACGCACGCGCGCACGTACACACACTCA2220.1909036968242912No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1970.16940553276750164Clontech SMART CDS Primer II A (100% over 21bp)
GTACATGGGGGGGGGCTTCCAAGCTGGTGCCCGTGGGCTACGGTATCCGG1830.1573665608956995No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGGACTCC1760.15134707495979843No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1640.14102795621253947No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1520.13070883746528048No Hit
GTACATGGGATCTTTAAAAAAATTTGGGGTCATTTATTGGGTATCTAGTG1480.12726913121619413No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1430.12296949840483623No Hit
GTACATGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGAC1390.11952979215574991No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1320.11351030621984882No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC1290.11093052653303406No Hit
TACACACACTCAGGCTTCAGATCTTGTTGAAAGCTGCGATATCGACACTC1280.1100705999707625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1280.1100705999707625No Hit
GTACATGGGGATAGAGGCCAGATTGTCTTGGAAGTTCCAAAGTTGCAACG1280.1100705999707625No Hit
CTTTACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC1260.10835074684621933RNA PCR Primer, Index 27 (95% over 21bp)
GTACATGGGCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACC1250.10749082028394776No Hit
GTACATGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA1240.10663089372167617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1200.10319118747258985No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA1190.10233126091031826No Hit
GAGTACATGGGGGGGGGCTTCCAAGCTGGTGCCCGTGGGCTACGGTATCC1180.10147133434804667No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)