FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001321930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116289
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9540.8203699404070893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8900.765334640421708No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4270.3671886420899655No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.1909036968242912Clontech SMART CDS Primer II A (100% over 21bp)
CACATATATCATGCAAGGTACAAATTTAAAAACATGTTACCACTAGATAC2040.17542501870340274No Hit
GTACATGGGGGGGGGCTTCCAAGCTGGTGCCCGTGGGCTACGGTATCCGG2030.17456509214113114No Hit
GTCTTTAATCGTGGCAGGGCCTCACGCACGCGCGCACGTACACACACTCA1830.1573665608956995No Hit
GTACATGGGATCTTTAAAAAAATTTGGGGTCATTTATTGGGTATCTAGTG1470.12640920465392255No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1450.12468935152937939No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1390.11952979215574991No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1390.11952979215574991No Hit
GTACATGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGGCCCTCGGAC1310.11265037965757724No Hit
TACACACACTCAGGCTTCAGATCTTGTTGAAAGCTGCGATATCGACACTC1200.10319118747258985No Hit
GTACATGGGGATAGAGGCCAGATTGTCTTGGAAGTTCCAAAGTTGCAACG1180.10147133434804667No Hit
GTACATGGGCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACC1170.10061140778577508No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)