FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001321936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436292
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT91242.0912599818470197Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT60331.3827895079442207Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT44191.0128537768283625Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC30880.707782861019684Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT25890.5934099181282261Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT25800.5913470794788811Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC25190.5773656175222099Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC24260.5560496181456456Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA22190.5086043292107121Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG21450.4916432114272093Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC21010.4815582224748563Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC18080.4144013642239601Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA16020.36718527958339825Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC14940.34243121579125907Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA14860.34059758143628577Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG13830.31698953911600486Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT12210.2798584434277961Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC11740.26908584159232807Clontech Universal Primer Mix Long (96% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10240.23470519743657917Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC9970.22851668148854437Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG9710.22255736983488122Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT8000.18336343549732748Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA7800.1787793496098943Clontech Universal Primer Mix Long (96% over 25bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG7720.176945715254921Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7200.16502709194759474No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6790.1556297158783567No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT6370.146003135514747Clontech SMART CDS Primer II A (96% over 26bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT5390.12354111466632439Clontech Universal Primer Mix Long (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)