FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001321937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857124
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT250252.919647565579776No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT138921.6207689902511189TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT65420.7632501248360797RNA PCR Primer, Index 1 (95% over 23bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG56530.6595311763525464TruSeq Adapter, Index 1 (96% over 28bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT22760.2655391751951876TruSeq Adapter, Index 1 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC19550.22808835127706142No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG15420.1799039578870735TruSeq Adapter, Index 1 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT14840.17313714235046504No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA13240.15447006500809685RNA PCR Primer, Index 1 (96% over 28bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAGGCAT10080.11760258725691966No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG9180.10710235625183753No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8890.10371894848353332No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC8750.10208557921607608Illumina PCR Primer Index 5 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)