FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001321945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757080
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT169402.2375442489565174No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT81401.0751836001479367TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT39660.5238548105880488RNA PCR Primer, Index 1 (95% over 23bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG30060.3970518307180219TruSeq Adapter, Index 1 (96% over 28bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT14270.1884873461193005TruSeq Adapter, Index 1 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC11370.15018227928356317No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA9660.12759549849421462RNA PCR Primer, Index 1 (96% over 28bp)
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG9190.12138743593807788TruSeq Adapter, Index 1 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT8750.11557563269403498No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7910.10448037195540762No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)