FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001321947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155635
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT115227.403219070260546No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT53453.434317473575995TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT29091.8691168438975807RNA PCR Primer, Index 1 (95% over 23bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG21551.3846499823304528TruSeq Adapter, Index 1 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12690.8153692935393709No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12200.7838853728274489No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT10320.6630899219327272TruSeq Adapter, Index 1 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC7290.46840363671410673No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG7030.4516978828669644TruSeq Adapter, Index 1 (95% over 24bp)
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA6560.421499020143284RNA PCR Primer, Index 1 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6490.4170013171844379No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT5840.375236932566582No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC3780.24287595977768497Illumina PCR Primer Index 5 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC3640.23388055385999293No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2730.1754104153949947Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG2680.1721977704243904No Hit
CTTCAAAATGTTTTTATTGGATTTAATTTCCCAAACACTGACATTTTAGA2390.15356442959488548No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2320.14906672663603945No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAGGCAT2300.14778166864779774No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2280.14649661065955602No Hit
GATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTG2230.14328396568895171No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2140.13750120474186397No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2020.12979085681241367No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG1710.10987245799466701No Hit
GTACATGGGAATTCAATTTGGCTTAGATTTGTGTTGCCTTTTTTTTCTTG1710.10987245799466701No Hit
GTACATGGGGGCATCGTGGTGGCAGATGTGCACATGGAGGGAACACAGAG1650.10601728402994184No Hit
GTATCAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATGAA1650.10601728402994184No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATATCGTATGCCGT1640.105374755035821No Hit
GTACATGGGAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATGAAA1580.10151958107109585No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1570.10087705207697498No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)