FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001321948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155635
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12050.7742474379156359No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9900.6361037041796511No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5530.35531853374883543No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG2340.15035178462428117No Hit
CTTCAAAATGTTTTTATTGGATTTAATTTCCCAAACACTGACATTTTAGA2330.1497092556301603No Hit
GATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTG2250.14456902367719343No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2080.13364603077713882No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.13107591480065536Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2040.13107591480065536No Hit
GTACATGGGAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATGAAA1850.11886786391235905No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1790.11501268994763389No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1760.1130851029652713No Hit
GTACATGGGAATTCAATTTGGCTTAGATTTGTGTTGCCTTTTTTTTCTTG1760.1130851029652713No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG1680.10794487101230443No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1660.1066598130240627No Hit
GTACATGGGGGATCACTTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAAC1620.10408969704757927No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1570.10087705207697498No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)