FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001321951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78087
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT1342317.189801119264413No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT65058.330451931819637RNA PCR Primer, Index 39 (95% over 23bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG44755.73078745501812RNA PCR Primer, Index 39 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT32284.133850704982904RNA PCR Primer, Index 39 (96% over 25bp)
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA16702.138640234610114RNA PCR Primer, Index 39 (96% over 30bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT12651.6199879621447872RNA PCR Primer, Index 39 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC9011.1538412283734807No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG7470.9566253025471588Illumina PCR Primer Index 11 (96% over 25bp)
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT7120.9118035012229948RNA PCR Primer, Index 39 (95% over 22bp)
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGC4210.539142238784945Illumina PCR Primer Index 11 (96% over 26bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCTCTCTA3990.5109685350954705No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC3580.45846299640144966No Hit
CTTATATACCATCTTTGACATCATTAAAAGTATCAAATCCCATTAATCTA3240.4149218179722617No Hit
TCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAA1840.2356346126756054Illumina PCR Primer Index 11 (96% over 29bp)
ATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTT1630.20874153188110695Illumina PCR Primer Index 11 (96% over 28bp)
CTTTACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC1280.1639197305569429RNA PCR Primer, Index 39 (95% over 21bp)
TCATTAGACAAAAAGCCTGAGGAACTGAAAGAACCATGTAAACATTTTCT1270.1626391076619668No Hit
ATCTATACCAAAATAGTCATCTTTCCTAACATTCCTCCCTTGATCTTCTG1230.1575166160820623No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACCTCTCTA1200.15367474739713397No Hit
CTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC1130.14471038713230114No Hit
CTATTGAATGCTTCTACTTAATTGTTTCATATAAAGGCTTTAACACAAAG1130.14471038713230114No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1130.14471038713230114No Hit
TCTTTAAGCATTTTGAAAGCAGAAAATGTTTACATGGTTCTTTCAGTTCC1080.13830727265742057No Hit
CATTAAAAGTATCAAATCCCATTAATCTAAAACTTCTTTAAGCATTTTGA1030.13190415818253998No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCTCTCT1010.12934291239258777No Hit
ATCTAAAACTTCTTTAAGCATTTTGAAAGCAGAAAATGTTTACATGGTTC1010.12934291239258777No Hit
GTTAATGACACACTATTGAATGCTTCTACTTAATTGTTTCATATAAAGGC890.11397543765287435No Hit
GCTTGAAACATAATAATTTTTCTGTATAAGCCACAGATCCTCTACCTCCT850.1088529460729699No Hit
GTTTCAAGCTGGGCACTTAATTCAAATCTTATCCTAAGTCACGCATCATT840.10757232317799377No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.10501107738804154No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACAACTCGTATGCCGT800.10244983159808932No Hit
GTCTAATGATGCGTGACTTAGGATAAGATTTGAATTAAGTGCCCAGCTTG800.10244983159808932No Hit
ATCCTAAGTCACGCATCATTAGACAAAAAGCCTGAGGAACTGAAAGAACC800.10244983159808932No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)