FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001321952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78087
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATACCATCTTTGACATCATTAAAAGTATCAAATCCCATTAATCTA2830.36241627927824094No Hit
CATTAAAAGTATCAAATCCCATTAATCTAAAACTTCTTTAAGCATTTTGA1280.1639197305569429No Hit
CTATTGAATGCTTCTACTTAATTGTTTCATATAAAGGCTTTAACACAAAG1220.1562359931870862No Hit
ATCTATACCAAAATAGTCATCTTTCCTAACATTCCTCCCTTGATCTTCTG1110.14214914134234893No Hit
TCATTAGACAAAAAGCCTGAGGAACTGAAAGAACCATGTAAACATTTTCT1040.1331847810775161No Hit
ATCCTAAGTCACGCATCATTAGACAAAAAGCCTGAGGAACTGAAAGAACC1040.1331847810775161No Hit
GTTTCAAGCTGGGCACTTAATTCAAATCTTATCCTAAGTCACGCATCATT1000.12806228949761164No Hit
ATCTAAAACTTCTTTAAGCATTTTGAAAGCAGAAAATGTTTACATGGTTC990.1267816666026355No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.12293979791770718No Hit
GTTAATGACACACTATTGAATGCTTCTACTTAATTGTTTCATATAAAGGC880.11269481475789825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.11013356896794602No Hit
TCTTTAAGCATTTTGAAAGCAGAAAATGTTTACATGGTTCTTTCAGTTCC840.10757232317799377No Hit
GTCTAATGATGCGTGACTTAGGATAAGATTTGAATTAAGTGCCCAGCTTG820.10501107738804154No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)