FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001321971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133217
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT1408710.57447623051112No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT66755.010621767492137RNA PCR Primer, Index 23 (95% over 23bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG46373.4807869866458487RNA PCR Primer, Index 23 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT34802.612279213613878RNA PCR Primer, Index 23 (96% over 25bp)
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA17981.3496775937005037RNA PCR Primer, Index 23 (96% over 30bp)
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT12750.9570850567119812RNA PCR Primer, Index 23 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC9060.6800933814753373No Hit
CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG7720.5795056186522741Illumina PCR Primer Index 1 (95% over 23bp)
TATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCT6770.508193398740401RNA PCR Primer, Index 23 (95% over 22bp)
ACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGC4200.31527507750512324Illumina PCR Primer Index 1 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4170.3130231126658009No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4170.3130231126658009No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCAGAGAG4030.30251394341563015No Hit
GTACAGGTTTTATTTATATGGCTCGAATTTTACAGTTTTCTTACTGCATC3780.2837475697546109No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCC3760.28224625986172935No Hit
ATATTGGATGGATCCTGAAGGTGAAATGAAACCAGGAAGAAAAGGTATTT3450.2589759565220655No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATATCGTATGCCGT3260.24471351253969087No Hit
AACTAGAACAGTACAGGTTTTATTTATATGGCTCGAATTTTACAGTTTTC3000.22519648393223088No Hit
CTTCTAAACAGAGCAGCAGCAGCAGAGATGATAACATGTTTCAGATTGGG2660.19967421575324468No Hit
GTTCTGGATTTAAAGAAATACCTTTTCTTCCTGGTTTCATTTCACCTTCA2520.18916504650307392No Hit
ACGTTAGTGTTCGCGATTTTAAAGGCAAAGTGCTAATTGATATTAGAGAA2410.18090784209222546No Hit
GTACATGGGGAGTCGCGAGCGAACGACCAAGAGGGTGTTAGACTGCTAGA2370.17790522230646238No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG2320.17415194757425856No Hit
GTAAAAAGACAGATTAAAACAACTAGAACAGTACAGGTTTTATTTATATG2320.17415194757425856No Hit
ATATTAGAGAATATTGGATGGATCCTGAAGGTGAAATGAAACCAGGAAGA2270.1703986728420547No Hit
ACATACAACAGCTTGGAGAACATTTAGAAAAGCCAATGTAAAAAGACAGA2220.16664539810985085No Hit
AACCTGTACTGTTCTAGTTGTTTTAATCTGTCTTTTTACATTGGCTTTTC2210.16589474316341007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2150.16139081348476547No Hit
GTTTAGAAGATGACAGGGCTCTCGAAGTCTCACCTGTCTTTTGTTTCTTT2120.15913884864544314No Hit
GTGCTAATTGATATTAGAGAATATTGGATGGATCCTGAAGGTGAAATGAA2040.153133609073917No Hit
GATTCTGACAGTGAGGTTGACAAAAAGAGGAAAAAGCAAGTTGCTCCAGA1960.14712836950239083No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA1910.14337509477018698No Hit
GTACATGGGAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAA1870.1403724749844239No Hit
GATTAAAACAACTAGAACAGTACAGGTTTTATTTATATGGCTCGAATTTT1870.1403724749844239No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT1800.13511789035933852No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT1800.13511789035933852No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT1770.1328659255200162No Hit
GTTTTCTTACTGCATCATCAATGTCAGAAATCTGTTCCTTCAGCTGGCTC1760.13211527057357544No Hit
ATCCAATATTCTCTAATATCAATTAGCACTTTGCCTTTAAAATCGCGAAC1730.12986330573425314No Hit
ATCCAGAACAATGGAGCCAGCTGAAGGAACAGATTTCTGACATTGATGAT1710.1283619958413716No Hit
GGTTTTATTTATATGGCTCGAATTTTACAGTTTTCTTACTGCATCATCAA1700.1276113408949308No Hit
ATACTACACTGTTACCCAGTGTGAAGGGAGCAGCCATGTGGCTCCCAAGC1670.1253593760556085No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATATCGTATGCCGTCTT1630.12235675626984543No Hit
GTACATGGGATTGCTCAGAACTTTGTGTGAGTTGAGCCCAGGCCTCTGGT1620.12160610132340467No Hit
CATTTAGAAAAGCCAATGTAAAAAGACAGATTAAAACAACTAGAACAGTA1590.11935413648408236No Hit
ATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTT1580.1186034815376416Illumina PCR Primer Index 1 (96% over 26bp)
AAAAAAAAGTATTCATCTTACAAATTCTTCTGCAATCCAAACATACAACA1560.11710217164476007No Hit
CATTAAAAAAAAGTATTCATCTTACAAATTCTTCTGCAATCCAAACATAC1550.11635151669831928No Hit
TATTTATATGGCTCGAATTTTACAGTTTTCTTACTGCATCATCAATGTCA1550.11635151669831928No Hit
GATGATAACATGTTTCAGATTGGGAAAATGAGGTACGTTAGTGTTCGCGA1540.1156008617518785No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA1540.1156008617518785No Hit
CACTAACGTACCTCATTTTCCCAATCTGAAACATGTTATCATCTCTGCTG1510.11334889691255622No Hit
CTTTACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC1490.11184758701967468RNA PCR Primer, Index 23 (95% over 21bp)
GTTCTAGTTGTTTTAATCTGTCTTTTTACATTGGCTTTTCTAAATGTTCT1490.11184758701967468No Hit
GTATTCATCTTACAAATTCTTCTGCAATCCAAACATACAACAGCTTGGAG1460.10959562218035236No Hit
GTGTTAGACTGCTAGAGCTGAGCGAAGCGATGCCTAAATCAAAGGAACTT1440.10809431228747082No Hit
GAACAGTACAGGTTTTATTTATATGGCTCGAATTTTACAGTTTTCTTACT1420.10659300239458928No Hit
AATAAAACCTGTACTGTTCTAGTTGTTTTAATCTGTCTTTTTACATTGGC1390.10434103755526697No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACCAGAGAG1340.10058776282306311No Hit
CTCTAATATCAATTAGCACTTTGCCTTTAAAATCGCGAACACTAACGTAC1340.10058776282306311No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)