FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001321972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133217
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4090.30701787309427475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3780.2837475697546109No Hit
GTACAGGTTTTATTTATATGGCTCGAATTTTACAGTTTTCTTACTGCATC3350.2514694070576578No Hit
ACATACAACAGCTTGGAGAACATTTAGAAAAGCCAATGTAAAAAGACAGA2990.2244458289857901No Hit
ATATTGGATGGATCCTGAAGGTGAAATGAAACCAGGAAGAAAAGGTATTT2950.22144320920002702No Hit
GTTCTGGATTTAAAGAAATACCTTTTCTTCCTGGTTTCATTTCACCTTCA2770.2079314201640932No Hit
GTTTAGAAGATGACAGGGCTCTCGAAGTCTCACCTGTCTTTTGTTTCTTT2700.2026768355390078No Hit
GTACATGGGGAGTCGCGAGCGAACGACCAAGAGGGTGTTAGACTGCTAGA2510.18841439155663314No Hit
AACTAGAACAGTACAGGTTTTATTTATATGGCTCGAATTTTACAGTTTTC2430.18240915198510702No Hit
GTAAAAAGACAGATTAAAACAACTAGAACAGTACAGGTTTTATTTATATG2420.18165849703866624No Hit
ACGTTAGTGTTCGCGATTTTAAAGGCAAAGTGCTAATTGATATTAGAGAA2370.17790522230646238No Hit
CTTCTAAACAGAGCAGCAGCAGCAGAGATGATAACATGTTTCAGATTGGG2340.17565325746714008No Hit
GTGCTAATTGATATTAGAGAATATTGGATGGATCCTGAAGGTGAAATGAA2260.16964801789561393No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2100.15763753875256162No Hit
ATATTAGAGAATATTGGATGGATCCTGAAGGTGAAATGAAACCAGGAAGA1970.1478790244488316No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA1940.1456270596095093No Hit
ATCCAATATTCTCTAATATCAATTAGCACTTTGCCTTTAAAATCGCGAAC1920.14412574971662775No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1900.14262443982374623No Hit
GTATTCATCTTACAAATTCTTCTGCAATCCAAACATACAACAGCTTGGAG1840.1381205101451016No Hit
GTACATGGGATTGCTCAGAACTTTGTGTGAGTTGAGCCCAGGCCTCTGGT1830.13736985519866082No Hit
AAAAAAAAGTATTCATCTTACAAATTCTTCTGCAATCCAAACATACAACA1800.13511789035933852No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA1800.13511789035933852No Hit
GGTTTTATTTATATGGCTCGAATTTTACAGTTTTCTTACTGCATCATCAA1800.13511789035933852No Hit
AACCTGTACTGTTCTAGTTGTTTTAATCTGTCTTTTTACATTGGCTTTTC1800.13511789035933852No Hit
ATCCAGAACAATGGAGCCAGCTGAAGGAACAGATTTCTGACATTGATGAT1780.133616580466457No Hit
GTAAGAAAACTGTAAAATTCGAGCCATATAAATAAAACCTGTACTGTTCT1770.1328659255200162No Hit
CATTAAAAAAAAGTATTCATCTTACAAATTCTTCTGCAATCCAAACATAC1760.13211527057357544No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT1760.13211527057357544No Hit
GTTTTCTTACTGCATCATCAATGTCAGAAATCTGTTCCTTCAGCTGGCTC1730.12986330573425314No Hit
GTTCTAGTTGTTTTAATCTGTCTTTTTACATTGGCTTTTCTAAATGTTCT1690.12686068594849007No Hit
GTACATGGGAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAA1680.1261100310020493No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT1610.1208554463769639No Hit
GATTAAAACAACTAGAACAGTACAGGTTTTATTTATATGGCTCGAATTTT1550.11635151669831928No Hit
TATTTATATGGCTCGAATTTTACAGTTTTCTTACTGCATCATCAATGTCA1540.1156008617518785No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT1540.1156008617518785No Hit
GATTCTGACAGTGAGGTTGACAAAAAGAGGAAAAAGCAAGTTGCTCCAGA1520.11409955185899696No Hit
GTACATGGGTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGACCGGAG1440.10809431228747082No Hit
GCCTAAATCAAAGGAACTTGTTTCTTCAAGCTCTTCTGGCAGTGATTCTG1440.10809431228747082No Hit
ATTTTTAATAATGCACATTAAAAAAAAGTATTCATCTTACAAATTCTTCT1440.10809431228747082No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG1430.10734365734103005No Hit
CTCTAATATCAATTAGCACTTTGCCTTTAAAATCGCGAACACTAACGTAC1410.10584234744814851No Hit
GTGTTAGACTGCTAGAGCTGAGCGAAGCGATGCCTAAATCAAAGGAACTT1360.10208907271594465No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT1340.10058776282306311No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)