FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001321973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245716
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT134625.478682706864836No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT66362.700678832473262RNA PCR Primer, Index 23 (95% over 23bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG39051.5892330983737324RNA PCR Primer, Index 23 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT31771.2929560956551467RNA PCR Primer, Index 23 (96% over 25bp)
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA16010.6515652216379886RNA PCR Primer, Index 23 (96% over 30bp)
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT11870.4830780250370347RNA PCR Primer, Index 23 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC8730.3552882189194029No Hit
CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG7950.3235442543424115Illumina PCR Primer Index 1 (95% over 23bp)
TATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCT7090.28854449852675446RNA PCR Primer, Index 23 (95% over 22bp)
GTTTTAAATCAGTGCATAAATTTTTCTTTTCTCATTTCAGCAGATGGACA6520.2653469859512608No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6240.2539517166159306No Hit
GAATAGACATAAACCAAAAAGAAGAAAAAAATCAGGATAAAGTGTCAACT5970.24296342118543357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5170.21040550879877581No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA4290.1745918051734523No Hit
ACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGC3870.15749890117045695Illumina PCR Primer Index 1 (95% over 24bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCAGAGAG3840.15627797945595728No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCC3440.1399990232626284No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC3420.13918507545296196No Hit
GTTTAATTATGATTCAGAAAAAATCGAGCGAATAACTTTCTCTGAAAAAA3210.1306386234514643No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATATCGTATGCCGT3210.1306386234514643No Hit
TCTGAGATGTTAGTATTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGC3100.12616191049829886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2970.12087124973546697No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT2920.11883638021130086No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA2900.1180224324016344No Hit
GTACATGGGATTTTTCTGATAACTTGTTCCTTTTCCTGACTCCTCCTTGC2790.11354571944846897No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA2770.11273177163880252No Hit
GTACATGGGTAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTC2600.10581321525663775No Hit
CCACAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAA2500.10174347620830552No Hit
GTATCAACGCAGAGTACATGGGATTTTTCTGATAACTTGTTCCTTTTCCT2470.10052255449380587No Hit
GACATAAACCAAAAAGAAGAAAAAAATCAGGATAAAGTGTCAACTATCAT2460.10011558058897263No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)