FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001321974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245716
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6360.25883540347392925No Hit
GAATAGACATAAACCAAAAAGAAGAAAAAAATCAGGATAAAGTGTCAACT6290.2559865861400967No Hit
GTTTTAAATCAGTGCATAAATTTTTCTTTTCTCATTTCAGCAGATGGACA6230.25354474271109734No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4890.1990102394634456No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA3820.15546403164629083No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC3450.14040599716746163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3080.1253479626886324No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA2830.11517361506780184No Hit
GTACATGGGTAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTC2820.11476664116296864No Hit
GTTTAATTATGATTCAGAAAAAATCGAGCGAATAACTTTCTCTGAAAAAA2770.11273177163880252No Hit
TCTGAGATGTTAGTATTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGC2710.11028992820980318No Hit
GTACATGGGATTTTTCTGATAACTTGTTCCTTTTCCTGACTCCTCCTTGC2710.11028992820980318No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT2550.10377834573247163No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA2540.1033713718276384No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA2480.10092952839863907No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)