FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001321975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151735
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT92216.077042211750749No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT41482.733713381882888RNA PCR Primer, Index 23 (95% over 23bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG29471.9422018650937491RNA PCR Primer, Index 23 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT24001.5817049461231754RNA PCR Primer, Index 23 (96% over 25bp)
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA12870.8481892773585527RNA PCR Primer, Index 23 (96% over 30bp)
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT9280.6115925791676278RNA PCR Primer, Index 23 (96% over 28bp)
CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG6240.4112432859920256Illumina PCR Primer Index 1 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5290.34863413187464987No Hit
TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC5270.3473160444195472No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4390.2893201963950308No Hit
TATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCT4020.26493557847563187RNA PCR Primer, Index 23 (95% over 22bp)
TTCCTAGGCCTTGTCAGCTTCAAGGCTTTAATTGAAGAATCTGAAATGAA3240.21353016772662864No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3110.20496259926846144No Hit
ACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGC3050.2010083369031535Illumina PCR Primer Index 1 (95% over 24bp)
GAATAAAGCTGATTTTAAAAGCACACAAAAGTTGAACACAAAGGAGAGGA2680.1766237189837546No Hit
GTCTAAGAAGGTTCTTCTAGGAGGCCCCGCCCCTCCAAATGGTCATTTCT2470.16278380070517678No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCAGAGAG2390.1575114508847662No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCC2330.15355718851945827No Hit
GTACATGGGACCCTGGATGATCAGTTTTCTGCTGAAGGCACCTACTCAGT2300.1515800573368043No Hit
GTACATGGGGTGTATGTATGCCAAGGAGACCGTGGGCTTTGGAATGCTCA2260.148943882426599No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATATCGTATGCCGT2230.14696675124394504No Hit
GCTTAGAATAAAGCTGATTTTAAAAGCACACAAAAGTTGAACACAAAGGA2140.14103535769598313No Hit
CCCTAGGAGGCCTGCCCCCGCTAACCGGCTTTTTGCCCAAATGGGCCATT2100.13839918278577784No Hit
TGTTAGGGTTGTACGGTAGAACTGCTATTATTCATCCTATGTGGGTAATT1930.12719543941740535No Hit
GGGTGTATGTATGCCAAGGAGACCGTGGGCTTTGGAATGCTCAAGGCCAA1930.12719543941740535No Hit
GGCTATAGCAATAAAACTAGGAATAGCCCCCTTTCACTTCTGAGTCCCAG1930.12719543941740535No Hit
GGTCCACAGAGGGGAAATAAAGTTGGAAATTTTTACTTTGAAAGTGACCT1760.11599169604903285No Hit
ATTTAAAGCATTGCAAACATTTGTAAGTTACATTTCTAGACAAGAACTGA1750.11533265232148153No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1730.11401456486637888No Hit
ATTGAGGAGTATGCTAAGATTTTGCGTAGCTGGGTTTGGTTTAATCCACC1680.11071934622862228No Hit
GTTTATTACTACTGGTATGAAATTCAGAGGAGACTGGTTTTGTCAGTTCT1670.11006030250107096No Hit
GTCTTGCTCTGTCATCTCAACTGGAGTGCAGTGGCATGGTCGTGGCTCAC1660.10940125877351961No Hit
GATTATAGTAGGTCCACAGAGGGGAAATAAAGTTGGAAATTTTTACTTTG1640.10808317131841697No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG1640.10808317131841697No Hit
GTATTATTAGATAGGTTATTTCCAGTTTTACTTCATGACAAATTACCTAG1630.10742412759086564No Hit
GCTTTAAATTATTAACAAATTTGTTGATTTGATTTTTGCAGGGTGGTGGC1610.106106040135763No Hit
GATTTATTTTTTTTTCCATTTCCAGTTTTTTAAAGTAGACACAGATTTGC1600.10544699640821167No Hit
GGATTTGGCAAAGATTTATTTTTTTTTCCATTTCCAGTTTTTTAAAGTAG1580.10412890895310904No Hit
ATCCTAACTACTACCGCATTCCTACTACTCAACTTAAACTCCAGCACCAC1560.1028108214980064No Hit
GGGTAGAATATCTTCCCCCTTATAAGTCACCAATTTCTGATCCCATTAAA1550.10215177777045507No Hit
TTATTAGATAGGTTATTTCCAGTTTTACTTCATGACAAATTACCTAGAGT1530.10083369031535243No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)