FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001321993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3177563
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT139440.4388268619693772No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT66430.2090595843418368RNA PCR Primer, Index 31 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60550.19055483715035704No Hit
ATATAGGCATGAGCCACTGAGCCTGGCCCAGAAGCGTTTTTCTCAAAGGC51640.1625144804367372No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50950.16034300500100235No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT36620.11524555138639266RNA PCR Primer, Index 31 (95% over 24bp)
GTCTTTAATCATTAATTGTCATATTTCTGATTTGTTAGCAAGTGCCAGCT34040.10712612149625358No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)