FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001322005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143902
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT90806.309849758863671No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT44253.0750093813845534TruSeq Adapter, Index 10 (95% over 21bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG26191.8199886033550612TruSeq Adapter, Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT25801.7928868257564174RNA PCR Primer, Index 10 (95% over 23bp)
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA11900.8269516754457895RNA PCR Primer, Index 10 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT9150.6358493975066364TruSeq Adapter, Index 10 (96% over 26bp)
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG5980.41556058984586736TruSeq Adapter, Index 10 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC5370.37317063001209155No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT3820.2654584369918417No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGC2910.20222095592834013Illumina Paired End PCR Primer 2 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC2470.17164459145807565No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGAGGCT2180.15149198760267404No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2150.14940723547970147No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATATCGTATGCCGT1850.1285597142499757No Hit
GGCCAGGCGTGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCCGA1850.1285597142499757No Hit
GACTAAGAGTGTCGGTGTGGAGGAGGGGGACCACCGATTTGGAGCAACGT1820.1264749621270031No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1790.12439021000403051No Hit
GGCTAACACGGTGAAACTAAAAATACAAAAAGTTAGCCGGGTGTGGTGGC1630.11327153201484343No Hit
GGCTGGAGTGCAGTGATGCGATCTCAACTCACTGCAACCTCCGCCTCCCA1520.10562744089727732No Hit
GCATTACCTGCTCAGGTGGACACGTTGCTCCAGATCGGTGGTCCCCCTGC1460.10145793665133215No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)