FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001322029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78554
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT1020612.992336481910533No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT48686.197010973343177RNA PCR Primer, Index 33 (95% over 22bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG30303.857219237721822RNA PCR Primer, Index 33 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT29733.784657687705273RNA PCR Primer, Index 33 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12021.5301575985945974No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT11571.4728721643710059RNA PCR Primer, Index 33 (96% over 27bp)
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA10651.355755276624997RNA PCR Primer, Index 33 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9821.250095475723706No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG8011.0196807291799272RNA PCR Primer, Index 15 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6720.8554624844056318No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC5900.7510756931537541No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5360.6823331720854444No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT4550.5792193904829799RNA PCR Primer, Index 33 (95% over 21bp)
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGC3630.46210250273697073RNA PCR Primer, Index 15 (96% over 25bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAAGAGGC2630.3348015377956565No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.32589047024976453No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC2390.3042493062097411No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGT2250.28642717111795707No Hit
GCTTTATTGAGTCCTTCTTTCTTGGGAGTAGCAGGCAATGCTTAGCAGGA2120.2698780456755862No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.23296076584260506No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1760.22404969829671306No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1720.21895765969906053Clontech SMART CDS Primer II A (100% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1650.21004659215316854No Hit
CTTCAATGGCTTTATTGAGTCCTTCTTTCTTGGGAGTAGCAGGCAATGCT1550.1973164956590371No Hit
ATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTT1400.17822135091783994RNA PCR Primer, Index 15 (96% over 27bp)
GTACATGGGTGATTACGGTGGATGGGAAGAGAGTCCCCAGAGATGCTGGA1250.1591262061766428No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1240.15785319652722968No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.15021513863075084No Hit
CTTTACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC1140.14512310003309825No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGTCTT1140.14512310003309825No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACAAGAGGC1010.1285739745907274No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA970.12348193599307483Clontech Universal Primer Mix Long (95% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.12220892634366169No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA940.1196629070448354No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG920.11711688774600912No Hit
CTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC910.11584387809659598No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC870.1107518394989434No Hit
GAGTACATGGAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCC810.10311378160246455No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA790.10056776230363826No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)