FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001322030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78554
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11471.4601420678768744No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8151.0375028642717112No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5930.7548947221019935No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5550.7065203554242941No Hit
GCTTTATTGAGTCCTTCTTTCTTGGGAGTAGCAGGCAATGCTTAGCAGGA2370.30170328691091475No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2260.28770018076737025No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1720.21895765969906053No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.2024085342566897No Hit
CTTCAATGGCTTTATTGAGTCCTTCTTTCTTGGGAGTAGCAGGCAATGCT1550.1973164956590371No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1450.18458639916490566No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1400.17822135091783994Clontech SMART CDS Primer II A (100% over 21bp)
GTACATGGGTGATTACGGTGGATGGGAAGAGAGTCCCCAGAGATGCTGGA1340.1705832930213611No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1310.16676426407312167No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA1130.14385009038368513Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1100.14003106143544566No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1100.14003106143544566No Hit
GTACATGGAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCT920.11711688774600912No Hit
GTATATGGGATAGGAGGACTACTCAAAACCATACCTCTCACTTCAACCTC880.11202484914835653No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA880.11202484914835653Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCC860.10947882984953027No Hit
GTATCAACGCAGAGTACATGGGTGATTACGGTGGATGGGAAGAGAGTCCC840.10693281055070397No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)