FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001322039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001322039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401307
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT101342.5252487497103218No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT48431.2068067589152445TruSeq Adapter, Index 3 (95% over 21bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG37990.946656798909563TruSeq Adapter, Index 3 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT27620.688251139402004RNA PCR Primer, Index 3 (95% over 23bp)
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA14820.3692933340310535RNA PCR Primer, Index 3 (96% over 28bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT11790.2937900410408989TruSeq Adapter, Index 3 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8360.208319316632902No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7390.18414829544463465No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG7170.17866620816482146TruSeq Adapter, Index 3 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC5760.14353101241692767No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT4560.11362871816340109No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4170.10391047253100494No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)